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Jyoti JaiswalAssociate Professor of Integrative Systems Biology
Associate Professor of Pediatrics (Secondary)
Office Phone: 202-476-6029
Department: Integrative Systems Biology
- BS, Allahabad University, India, 1993
- M.S., M.S. University of Baroda, India, 1995
- PhD, Indian Institute of Science, 2001
Even in our most idle moments, cells in our body are busily at work synthesizing molecules and transporting them within and outside their boundary. This allows communication and thus coordination between activities of the compartments in our cells and the cells in our body. This coordination in turn maintains our body in a functional state. In undergraduate and graduate work I used rice plant and a simple eukaryote (Dictyostelium) as models to study the role of chemical and genetic factors in maintaining the functional state of an organism during growth, development and stress. In my postdoctoral work I studied how this communication occurs in cells in our body. I developed and used tools for visualizing individual cellular and molecular processes in live cells and applied them to study the mechanisms and the machinery used by our cells for regulated trafficking of molecules. In 2005 when I started my independent research career at The Rockefeller University, I focused my efforts on understanding the relevance of inter- and intra-cellular communication to human diseases. To pursue this interest further, in 2009 I moved my lab to the Children’s National Medical Center (CNMC) and joined the department of Integrated Systems Biology at George Washington University. Here the work I am pursuing combines the use of cell imaging and ‘omics’-based approaches to study the machinery for intracellular trafficking and secretion (exocytosis) and decipher its role in degenerative diseases and healing of tissue injury. At CNMC I also direct the ‘Cellular Imaging Core’ and through this extend support to various laboratories in design, performance and analysis of cell and tissue imaging studies.
The Rockefeller University, NY Post-doc 2004 Cell biology; Optical imaging
- Exocytosis of post-Golgi vesicles is regulated by components of the endocytic machinery. Jaiswal JK, Rivera VM, Simon SM. Cell. 2009: 137(7):1308-19.PMID: 19563761
- Resolving vesicle fusion from lysis to monitor calcium-triggered lysosomal exocytosis in astrocytes. Jaiswal JK, Fix M, Takano T, Nedergaard M, Simon SM. Proc Natl Acad Sci U S A. 2007: 104(35):14151-6. PMID: 17715060
- Imaging single events at the cell membrane. Jaiswal JK, Simon SM. Nat Chem Biol. 2007: 3(2):92-8. PMID: 17235347
- Patients with a non-dysferlin Miyoshi myopathy have a novel membrane repair defect. Jaiswal JK, Marlow G, Summerill G, Mahjneh I, Mueller S, Hill M, Miyake K, Haase H, Anderson LV, Richard I, Kiuru-Enari S, McNeil PL, Simon SM, Bashir R. Traffic. 2007: 8(1):77-88. PMID: 17132147
- Use of quantum dots for live cell imaging. Jaiswal JK, Goldman ER, Mattoussi H, Simon SM. Nat Methods. 2004: 1(1):73-8. PMID: 16138413
- Tracking metastatic tumor cell extravasation with quantum dot nanocrystals and fluorescence emission-scanning microscopy. Voura EB, Jaiswal JK, Mattoussi H, Simon SM. Nat Med. 2004: 10(9):993-8. PMID: 15334072
- Synaptotagmin VII restricts fusion pore expansion during lysosomal exocytosis. Jaiswal JK, Chakrabarti S, Andrews NW, Simon SM. PLoS Biol. 2004: 2(8): E233. PMID: 15226824
- Imaging single membrane fusion events mediated by SNARE proteins. Fix M, Melia TJ, Jaiswal JK, Rappoport JZ, You D, Söllner TH, Rothman JE, Simon SM. Proc Natl Acad Sci U S A. 2004: 101(19):7311-6. PMID: 15123811
- Long-term multiple color imaging of live cells using quantum dot bioconjugates. Jaiswal JK, Mattoussi H, Mauro JM, Simon SM. Nat Biotechnol. 2003: 21(1):47-51. PMID: 12459736
- Membrane proximal lysosomes are the major vesicles responsible for calcium-dependent exocytosis in nonsecretory cells. Jaiswal JK, Andrews NW, Simon SM. J Cell Biol. 2002: 159(4):625-35. PMID: 12438417
Cellular and molecular analysis of regulated secretion: Membrane bound compartments called vesicles are used by our cells for regulated (calcium-triggered) and constitutive secretion of proteins and lipids. A growing list of functions is being attributed to the regulated secretory ability of cells in our body. However, the identity of many of the compartments and the molecular machinery responsible for trafficking and regulated secretion is not known. We are working to identify such compartments and the mechanism that regulate their trafficking and exocytosis in muscle, astroglial, lung and immune cells. Lysosomes and related organelles are ubiquitous regulated exocytic compartments involved in functioning of our cells and are a key target of our analysis.
Diseases with intracellular trafficking and secretion defects: Tissue damage by factors including pathogenic, environmental and mechanical compromise the integrity of the tissue and individual cells in it. Thus healing requires (A) repairing the damaged cell membrane to prevent death of the injured cells and (B) recruiting inflammatory and immune cells to remove any cells that failed to heal and also initiate the process of regeneration. Fusion of intracellular vesicles at the site of wound is required for healing the plasma membrane of the injured cells as well as for secreting signals to attract the inflammatory and immune cells. Mutations leading to a defect in vesicle trafficking and fusion causes progressive accumulation of un-repaired tissue lesions resulting in degenerative and inflammatory disease. Miyoshi Myopathy / Limb Girdle Muscular Dystrophy 2B are examples of such genetic diseases that affect muscle. In addition, defects in trafficking and exocytosis of lysosomes and lysosome related organelles result in diseases such as Chediak Higashi, Hermansky Pudlak and Griscelli syndromes. In collaboration with clinicians and geneticists we are trying to decipher the link between the clinical and cellular defects in these and other diseases.
Innovative technologies: We are interested in developing and improving existing tools and technologies to image individual events across the biological scale from molecular to organismal. For this we are working to develop probes that allow multimode imaging at these scales. As cellular communication involves changes in level and activity of proteins and small molecules, probes to monitor these changes in live cells are another tool we aim to develop. In addition, together with other labs we are employing novel tools for mouse genetic manipulation and membrane proteomics to study the pathways for healing injury from cellular to tissue level.
Link to publications on Pubmed: http://www.ncbi.nlm.nih.gov/pubmed/?term=jaiswal+jk
View publications by this faculty member from January 1, 2013 - present
Additional publications published before January 1, 2013 may be available within Himmelfarb Library's database.