Center for microscopy and image analysis (CMIA) The George Washington University Office of the VICE president for research Director: Anastas Popratiloff, MD, PhD, anaasp@gwumc.edu, Ph: +1.202.994.2881, 2300 I Street NW, Ross HAll 406, Washington DC 20037

Center for microscopy and image analysis (CMIA)
The George Washington University
Office of the VICE president for research

Director: Anastas Popratiloff, MD, PhD,
anaasp@gwumc.edu, Ph: +1.202.994.2881, 2300 I Street NW, Ross HAll 406, Washington DC 20037

For schedule login to FOM: https://jboss.gwumc.edu/fom/welcome

The Center for Microscopy and Image Analysis (CMIA) at George Washington University (GW) provides university-wide core resources for acquisition, enhancement and analysis of microscopic images of relevance to biological, biomedical, translational and engineering sciences. The CMIA was made an institutional core facility in 1999 by a commitment of considerable institutional support. CMIA has remained true to its original goals of research, dissemination of knowledge, and educational opportunities at several levels. CMIA is supported by the GW - Office of the Vice President for Research. Among the high priorities of the CMIA are maximizing the utilization of the available equipment and generation of highest quality scientific data. In this respect, CMIA will not only provide new and existing investigators with proper training on basic operation of the instrumentation, but will also focus on tailored training and advice aiming the achievement of highest quality image data acquisition, interpretation and analysis. CMIA is dedicated to identify and utilize extra budgetary recourses for equipment upgrades and acquisition of new generation instrumentation and its rapid and effective integration into the existing administrative and training structure. BACKGROUND OF CMIA In 1993, the Electron Microscopy Laboratory affiliated with the Department of Anatomy and Cell Biology was renamed Center for Microscopy and Image Analysis (CMIA) to reflect the changing technologies of contemporary science. Thus, the CMIA provided instrumentation for biomedical imaging and analysis applications. Under the direction of Dr. Robyn Rufner, equipment acquisitions and renovations to the 1900 sq. ft. facility in 1997 expanded the technologies available for members of the GW Medical Center research community. In 1999, the CMIA was designated a GWUMC Core Facility affiliated with the Office of Health Research, Compliance and Technology Transfer. Over 100 faculty, staff and graduate students have been trained each year to effectively utilize CMIA instrumentation and workstations to generate valuable data for their scientific research. CMIA is integral component of the Cellular Neuroimaging Core of Intellectual and Developmental Disabilities Research Center (IDDRC) at Children National Medical Center (CNMC). This affiliation provided structured financial support for essential upgrades of the CMIA and streamlined the access of researchers from CNMC to the infrastructure at the GW.
Over the past two years the Center has rapidly expanded, reflecting the global revitalization of the microscopic imaging, triggered by life-cell and tissues capabilities on the modern instruments. Thus with the help of internal and external extra-budgetary financial support, the Center itemized over one million in new equipment and upgrades.

EXTERNAL USERS CMIA offers available instrumentation to the external users for fee. There are several possible scenarios for such collaborations. For more information, please contact CMIA director at anaasp@gwumc.edu AVAILABLE TECHNOLOGY (outline)
- Zeiss 510 confocal system with CO2/temperature control and scanning stage
- Zeiss 710 confocal system with CO2/temperature control and scanning stage
- Bio-Rad MRC 1024 ES Confocal Laser Scanning Microscope, Kr/Ar laser, three PMT and Olympus IX-70 Inverted Fluorescence Microscope. - Bio-Rad MRC 1024 ES Confocal Laser Scanning Microscope, Kr/Ar laser, two PMT and NIKON ECLIPSE TE200 Inverted Fluorescence Microscope, equipped with microinjection system.
- Microscope Workstation with an Olympus BX-60 light/fluorescence microscope, including a Ludl focus motor, an Evolution Digital CCD camera and Image-Pro Plus software. - NIKON Eclipse TE300 inverted fluorescence microscope with Optronics CCD camera. - Image Analysis Workstations with Image-Pro Plus and MetaMorph software on Dell 7400 and 64 bit Windows OS.
- Volocity software on iMAc - A JEOL JEM-1200EX Transmission Electron Microscope (TEM), equipped with SIA-CAM, 4MP cooled CCD camera. - Ultramicrotome Workstations for sectioning specimens. - An Amersham Biosciences Storm 860 Phosphor Imager - Densitometer SI Workstation with ImageQuant software - Fluor-S MultiImager - Konica Film processor .

CMIA MISSION