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 publications, contact information Research Interests My research interests focus around the hookworm infectious process. The developmentally arrested infective stage receives a host-specific signal that re-initiates suspended developmental pathways that culminate in development of the adult parasite. I am investigating the molecular events of this activation to parasitism in the framework of dauer recovery in the free-living nematode Caenorhabditis elegans. Using a novel in vitro activation assay I developed, I have confirmed that hookworm infective larvae use the same signaling pathways during activation that C. elegans dauers use during recovery. I am continuing to dissect the pathways in hookworm larvae by identifying the host-specific signal and its receptor, and the molecules involved in the transduction of this signal and the molecular events of development. I am also interested in the population genetics of natural populations of hookworms infecting humans in China. I have an ongoing NIH- funded project examining the genetic structure of hookworm populations and molecular variation in candidate vaccine antigens. These studies have demonstrated the presence of genetically isolated populations of the human hookworm Necator americanus in China. Variation in these populations could complicate the design of recombinant vaccines and effective drug-based control strategies. Future studies will investigate the effect of transmission patterns on the intra- and interhost population genetics of hookworms in China. Selected Publications Top Murrell, K.D. and Hawdon, J.M. 2009. In memoriam: Gerry A. Schad. J Parasitol. [Epub ahead of print] . Wang, Z., Zhou, X.E., Motola, D.L., Gao, X., Suino-Powell, K., Conneely, A., Ogata, C., Sharma, K.K., Auchus, R.J., Lok, J.B., Hawdon, J.M., Kliewer, S.A., Xu, H.E., Mangelsdorf, D.J., 2009. Identification of the nuclear receptor DAF-12 as a therapeutic target in parasitic nematodes. Proceedings of the National Academy of Sciences U S A, online before print June 2, 2009. Kiss, J.E., Gao, X., Krepp, J.M. and Hawdon, J.M. 2009. Interaction of hookworm 14-3-3 with the forkhead transcription factor DAF-16 requires intact Akt phosphorylation sites. Parasites & Vectors, 2: 21. Wolpert, B.J., Beauvoir, M.G., Wells, E.F., and Hawdon, J.M. 2008. Plant vermicides of Haitian Vodou show in vitro activity against larval hookworms. Journal of Parasitology 94:1155-1160. Abubucker, S., Martin, J., Yin, Y., Fulton, L., Yang, S.-P., Hallsworth-Pepin, K., Johnston, J. S., Hawdon, J., McCarter, J. P., Wilson, R. K. and Mitreva, M., 2008. The canine hookworm genome: Analysis and classification of Ancylostoma caninum survey sequences. Molecular and Biochemical Parasitology 157: 187-192. Mitreva, M., J. P. McCarter, P. Arasu, J. M. Hawdon, J. Martin, M. Dante, T. Wylie, J. Xu, J. E. Stajich, W. Kapulkin, S. W. Clifton, R. H. Waterston, and R. K. Wilson. 2005. Investigating hookworm genomes by comparative analysis of two Ancylostoma species. BMC Genomics 6: 58. Delaney, A., A. Williamson, A. Brand, J. Ashcom, G. Varghese, G. N. Goud, and J. M. Hawdon. 2005. Cloning and characterisation of an aspartyl protease inhibitor (API-1) from Ancylostoma hookworms. Inter. J. Parasitol. 35: 303-313. Zhan, B., Y. Wang, Y. Liu, A. Williamson, A. Loukas, J. M. Hawdon, H.-c. Xue, S.-h. Xiao, and P. J. Hotez. 2004. Ac-SAA-1, an immunodominant 16 kDa surface-associated antigen of infective larvae and adults of Ancylostoma caninum. Int J Parasitol 34: 1037-1045. Li, T. H., X. R. Guo, J. Xue, L. Hu, H. Q. Qiang, H. C. Xue, Z. Bin, J. M. Hawdon, and S. H. Xiao. 2004. Comparison of mitochondrial cytochrome oxidase 1 DNA sequences from Necator americanus hookworms maintained for 100 generations in golden hamsters (Mesocricetus auratus) and hookworms from natural human infections. Acta Trop 92: 71-75. Brand, A., and J. M. Hawdon. 2004. Phosphoinositide-3-OH-kinase inhibitor LY294002 prevents activation of Ancylostoma caninum and Ancylostoma ceylanicum third-stage infective larvae. Int. J. Parasitol. 34: 909-914. Hawdon, J. M., and B. Datu. 2003. The second messenger cyclic GMP mediates activation in Ancylostoma caninum infective larvae. Int. J. Parasitol. 33: 787-793. Hawdon, J. M., B. Datu, and M. Crowell. 2003. Molecular cloning of a novel multidomain Kunitz-type proteinase inhibitor from the hookworm Ancylostoma caninum. J Parasitol 89: 402-407. Zhan, B., P. J. Hotez, Y. Wang, and J. M. Hawdon. 2002. A developmentally regulated metalloprotease secreted by host-stimulated Ancylostoma caninum third-stage infective larvae is a member of the astacin family of proteases. Mol Biochem Parasitol 120: 291-296. Hawdon, J. M., T. Li, B. Zhan, and M. S. Blouin. 2001. Genetic structure of populations of the human hookworm, Necator americanus, in China. Mol Ecol 10: 1433-1437. Zhan, B., T. Li, S. Xiao, F. Zheng, and J. M. Hawdon. 2001. Species-specific identification of human hookworms by PCR of the mitochondrial cytochrome oxidase I gene. J Parasitol 87: 1227-1229. Qiang, S., Z. Bin, X. Shu-hua, F. Zheng, P. Hotez, and J. M. Hawdon. 2000. Variation between ASP-1 molecules from Ancylostoma caninum in China and the United States. J Parasitol 86: 181-185. Tissenbaum, H. A., J. M. Hawdon, M. Perregaux, P. Hotez, L. Guarente, and G. Ruvkun. 2000. A common muscarinic pathway for diapause recovery in the distantly related nematode species Caenorhabditis elegans and Ancylostoma caninum. Proc. Natl. Acad. Sci. USA 97: 460-465. Hawdon, J. M., S. Narasimhan, and P. J. Hotez. 1999. Ancylostoma secreted protein 2 : cloning and characterization of a second member of a family of nematode secreted proteins from Ancylostoma caninum. Mol. Biochem. Parasitol. 99: 149-165 Hawdon, J. M. 1996. Differentiation between the human hookworms Ancylostoma duodenale and Necator americanus using PCR-RFLP. J. Parasitol. 82: 642-647. Hawdon, J. M., B. F. Jones, D. R. Hoffman, and P. J. Hotez. 1996. Cloning and characterization of Ancylostoma secreted protein: a novel protein associated with the transition to parasitism by infective hookworm larvae. J. Biol. Chem. 271: 6672-6678. . Hawdon, J. M., and S. A. Johnston. 1996. Hookworm in the Americas: an alternative to trans-Pacific contact. Parasitol. Today 12: 72-74. Hawdon, J. M., and P. J. Hotez. 1996. Hookworm: developmental biology of the infectious process. Curr. Opin. Gen. Dev. 6: 618-623. Hawdon, J. M., B. F. Jones, M. A. Perregaux, and P. J. Hotez. 1995. Ancylostoma caninum: metalloprotease release coincides with activation of infective larvae in vitro. Exp. Parasitol. 80: 205-211. Hawdon, J. M., B. F. Jones, and P. J. Hotez. 1995. Cloning and characterization of a cDNA encoding the catalytic subunit of a cAMP-dependent protein kinase from Ancylostoma caninum third-stage infective larvae. Mol. Biochem. Parasitol. 69: 127-130. Complete publication list here Contact Information Top John M. Hawdon , Ph.D. |
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