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 publications, contact information Research Interests My research interests are the discovery, expression, purification and process development of antigens as vaccine candidates for testing in pre-clinical and clinical trials. I am now serving as the Director of the Protein Expression, Purification and Process Development group of the Human Hookworm Vaccine Initiative (HHVI), sponsored by Sabin Vaccine Institute and located at the George Washington University (GWU). My lab has expertise in expression of proteins in Pichia pastoris and E. coli and their subsequent purification using various chromatographic techniques. My lab has successfully developed processes for the expression and purification of leading human hookworm vaccine candidate Na -ASP-2 for cGMP manufacturing. My lab successfully transferred the technology to WRAIR and oversaw cGMP manufacturing of Na-ASP-2, which is in Phase-I clinical trials. My lab is currently involved in process optimization for re-manufacturing of Na-ASP-2. I am also interested in discovery of antigens of infectious diseases and cancer. I am applying phage display technology for the identification of protein biomarkers/antigens of HCV-associated liver cancer and HIV-1 promoted hepatocellular carcinoma, along with other collaborators at GWU. Selected Publications Top Jeffrey Bethony, Alex Loukas, Michael Smout, Simon Brooker, Susana Mendez, Jordan Plieskatt, Gaddam Goud, Maria Elena Bottazzi, Bin Zhan,Yan Wang, Angela Williamson, Sara Lustigman, Rodrigo Correa-Oliveira, Shuhua Xiao, and Peter J. Hotez (2005). Antibodies against a secreted protein from hookworm larvae reduce the intensity of hookworm infection in humans and vaccinated laboratory animals. The FASEB Journal, 2005 (In Press). Bin Zhan, Sen Liu, Samirah Perally , Jian Xue, Ricardo Fujiwara, Peter Brophy, Shuhua Xiao , Yueyuan Liu, Jianjun Feng, Angela Williamson, Yan Wang, Lilian L. Bueno, Susana Mendez, Gaddam Goud, Jeffrey M. Bethony, John M. Hawdon, Alex Loukas, Karen Jones and Peter J. Hotez (2005). Biochemical characterization and vaccine potential of a heme binding glutathione transferase (GST) from the adult hookworm Ancylostoma caninum. Infection and Immunity, 2005 (In Press). Alex Loukas, Jeffrey M. Bethony, Susana Mendez, Ricardo T. Fujiwara, Gaddam Narsa Goud, Najju Ranjit, Bin Zhan, Karen Jones, Maria Elena Bottazzi, Peter J. Hotez (2005). Vaccination with recombinant aspartic hemoglobinase reduces parasite load and blood loss after hookworm infection. PLoS Medicine, 2005 (In Press). Gaddam Narsa Goud , Maria Elena Bottazzi, Bin Zhan, Susana Mendez, Vehid Deumic, Jordan Pleiskatt, Sen Liu, Yan Wang, Lillian Bueno, Ricardo Fujiwara, Andre Samuel, So Yeong Ahn, Maneesha Solanki, Oluwatoyin A. Asojo, Jin Wang, Jeffrey M. Bethony, Alex Loukas, Michael Roy, Peter J. Hotez (2005). Expression of the Necator americanus hookworm larval antigen Na-ASP-2 in Pichia pastoris and purification of the recombinant protein for use in human clinical trials. Vaccine, 2005, 15; 23(39):4754-64. Susana Mendez, Bin Zhan, Gaddam Goud, Kashinath Ghosh, Azra Dobardzic, Wei-hua Wu, Sen Liu, Vehid Deumic, Reshad Dobardzic, Yueyuan Liu, Jeff Bethony and Peter J. Hotez. Effect of combining the larval antigens Ancylostoma Secreted Protein 2 (ASP-2) and metalloprotease-1 (MTP-1) in protecting hamsters against hookworm infection and disease caused by Ancylostoma ceylanicum. Vaccine, 2005, 23, 3123–3130. Oluwatoyin A. Asojo, Gaddam Goud, Kajari Dhar, Alex Loukas, Bin Zhan, Vehid Deumic, Sen Liu, Gloria E. O. Borgstahl and Peter J. Hotez. Novel X-ray structure of Na-ASP-2, a Pathogenesis Related-1 protein from the nematode parasite, Necator americanus , and a vaccine antigen for human hookworm infection. J Mol Biol, 2005, 25; 346(3):801-14. Angela Delaney, Angela Williamson, Andrea Brand, James Ashcom, Geeta Varghese, Gaddam Narsa Goud, John M. Hawdon. Cloning and characterization of an aspartyl protease inhibitor (API-1) from Ancylostoma hookworms International Journal for Parasitology, 2005, 35, 303–313. Ruitian Liu, Bin Yuan, Andleeb Zameer, Philip Schulz, Chad McAllister, Yuri Lyubchenko, Gaddam Goud and Michael R. Sierks. Single chain variable fragments against β-amyloid (Aβ) inhibit Aβ aggregation and prevent Aβ-induced neurotoxicity. Biochemistry, 2004, 43, 6959-67. Alex Loukas, Jeffrey M. Bethony, Angela L. Williamson, Gaddam N. Goud, Susana Mendez, Bin Zhan, John M. Hawdon, Maria Elena Bottazzi, Paul J. Brindley, Peter J. Hotez. Vaccination of dogs with a recombinant cysteine protease from the intestine of canine hookworms diminishes fecundity and growth of worms. The Journal of Infectious Diseases, 2004, 189, 1952-61. Gaddam Narsa Goud, Bin Zhan, Kashinath Ghosh, Alex Loukas, John Hawdon, Azra Dobardzic, Vehid Deumic, Sen Liu, Reshad Dobardzic, Bernard C. Zook, Qun Jin, Yueyuan Liu, Laura Hoffman, Sophia Chung-Debose, Rachna Patel, Susana Mendez, and Peter J. Hotez . Cloning, Yeast Expression, Isolation, and Vaccine Testing of Recombinant Ancylostoma-Secreted Protein (ASP) -1 and ASP-2 from Ancylostoma ceylanicum. The Journal of Infectious Diseases, 2004, 189, 919-929. Hotez PJ, Zhan B, Bethony JM, Loukas A, Williamson A, Goud G, Hawdon JM, Dobardzic A, Dobardzic R, Ghosh K, Bottazzi ME, Mendez S, Zook B, Wang Y, Liu S, Essiet-Gibson I, Chung-Debose S, Xiao SH, Knox D, Meagher M, Inan M, Correa-Oliveira R, Vilk P, Shepherd HR, Brandt W, Russell PK. Progress in the development of a recombinant vaccine for human hookworm disease: the human hookworm vaccine initiative. International Journal for Parasitology, 2003, 33(11), 1245-1258. Gaddam Narsa Goud, Olga Artsaenko, Mikael Bols and Michael Sierks (2001). Specific Carbohydrase Activity isolated from a Phage Display Antibody Library. Biotechnology Progress, 2001, 17 (1), 197-202. Huiping Hu, Gaddam Narsa Goud, and Michael R. Sierks (1998). Artificial Antibodies for affinity Chromatography of homologous proteins: Application to blood clotting proteins. Biotechnology Progress, 1998, 14, 496-499. Anshuman V. Patwardhan, Gaddam Narsa Goud, Richard Koepsel, and Mohammad M. Ataai (1998). Phage displayed libraries for the selection of optimal affinity tails for protein purification using Ni-NTA chromatography. Biotechnology Techniques, 1998, 2 (6), 421-424. Anshuman V. Patwardhan, Gaddam Narsa Goud, Richard Koepsel, and Mohammad M. Ataai. Selection of optimum affinity tags from a phage-displayed peptide library: Application to immobilized copper (II) affinity chromatography. Journal of Chromatography, 199, A, 787:1-2, 91-100. Gaddam Narsa Goud, Anshuman V. Patwardhan, Eric J. Beckman, Mohammad M. Ataai, and Richard Koepsel . Selection of specific peptide ligands for immobilized metals using a phage displayed library: Application to protein separation using IMAC. International Journal of Biochromatography, 1997, 3 (2), 123-138. Badar Taqui Khan, K. Murali Mohan, S. Rounaq Ali Khan and G. Narsa Goud. Synthesis and characterization of platinum (II) and palladium (II) complexes of methionine and nucleosides. Indian J. Chem., 1995, 34A, 573-575. Badar Taqui Khan, G. Narsa Goud, and K. Murali Mohan. Mixed ligand complexes of palladium (II) & platinum (II) with methionine and purines. Indian J. Chem 31A, 1992, 106-109. Badar Taqui Khan, G. Narsa Goud, S. Vijaya Kumari and K. Murali Mohan. Mixed ligand complexes of platinum (II) with histidine as primary ligand and purines, pyrimidines and nucleosides as secondary ligands. Indian J. Chem, 1992, 31A, 28-33. Badar Taqui Khan, G. Narsa Goud and K. Murali Mohan. Mixed ligand complexes of palladium (II) & platinum (II) with methionine and 2, 4-disubstituted pyrimidines. Transition Metal Chemistry, 1990, 15, 407-410. Badar Taqui Khan, S. Vijaya Kumari, K. Murali Mohan and G. Narsa Goud. Complexes of platinum (II & IV) with disubstituted purines. Polyhedron, 1985, 4, 1617-1619. Badar Taqui Khan, G. Narsa Goud and S. Vijaya Kumari (1983). Interaction of platinum (II) amino acid complexes with nucleosides. Inorg. Chim. Acta, 1983, 80, 145-149. Badar Taqui Khan, S. Vijaya Kumari and G. Narsa Goud (1982). Mixed ligand complexes of platinum (II) with amino acids and purines and pyrimidines. J. Coord. Chem, 1982, 12, 19-25. adar Taqui Khan, S. Vijaya Kumari and G. Narsa Goud (1982). Complexes of platinum (II & IV) with adenine and guanine. Indian J. Chem, 1982, 21A, 264-267. Contact Information Top Gaddam Narsa Goud, Ph. D |
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