FACSAria

A high-speed sorter with a fixed-alignment cuvette flow cell from BD Biosciences, located in Ross Hall 416. Three lasers provide excitation wavelengths of 488 nm, 633 nm and 407 nm. Capable of detecting up to 9 fluorochromes, and sorting (up to 25,000 events per second for lymphocytes; 5,000-8,000 events per second for larger cells) four populations simultaneously. Capable of sorting into multiwell plate or onto slide.

Fee for the FACSAria sorter: $100/hr. The sorter is operated by Core Facility personnel. Investigators will be charged on an hourly basis. Please contact Teresa for request forms.

FACSCalibur

An analyzer with fixed-alignment cuvette flow cell from BD Biosciences, located in Ross Hall 418. Two lasers provide excitation wavelengths of 488 nm and 635 nm. Capable of detecting up to 4 fluorochromes simultaneously.

Fee for the FACSCalibur analyzer: $25/hr. New users are required to complete a 2-hour training session. Usage of the analyzer requires password log-in. Individual usage charts keep track of the actual time used by each user.

Basic hands-on training for beginners: no charge. Advanced training for special applications: $25/hr.

Macintosh Workstation

Equipped with CellQuest analysis software from BD Biosciences, located in Ross Hall 418. Useful for analysis of data generated on the FACSCalibur. It is available to all investigators who utilize the Flow Cytometry Core Facility.

PC Workstation

Equipped with WinList 3D v6.0 analysis software from Verity Software House, located in Ross Hall 416A. Useful for analysis of data generated on the FACSAria. It is available to all investigators who utilize the Flow Cytometry Core Facility.