George Washington University Medical Center
Flow Cytometry Core Facility

Ross Hall 118

2300 Eye Street NW, Washington, DC 20037

 

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Multiparameter Flow Cytometry


Microscopic image of fluorescent proteins Flow cytometric analysis of 10 populations Isometric 3D view of 6 populations

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  • Microscopic image of cells expressing individual fluorescent protein genes, illustrating the high degree of spectral overlap
  • Flow cytometry compensates for the spectral overlap and readily discriminates 10 populations of cells expressing various combinations of fluorescent protein genes
  • Accurate spectral overlap compensation is easily assessed with the new HyperLog transform which preserves the symmetry of measurement variability

Logarithmic transform display

  • With the traditional logarithmic transform, symmetry is not preserved as events plotted on the axis are not easily visualized

 

Biexponential transform display

  • The Biexponential transform is another new transform that displays properly compensated populations as symmetric distributions


FCOM histogram display

  • FCOM histogram facilitates visualization of multiple populations within a mixture

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© 2006 The George Washington University Medical Center; All rights reserved.
Last Updated: March 8, 2006