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The George Washington
University is offering several tracks to the MS degree in Genomics and
Bioinformatics also utilizes the McCormick genomics Center. The
McCormick Center was designed to promote the research, education and
clinical practice in the genomics of human disease and includes a core
facility to coordinate and facilitate gene-based research. Equipment
currently available at the McCormick core facility includes.
MS Students are given two
part-time assistant-ships a year which also allows for nine credit hour
tuition break. Also, almost all second-year students do usually obtain
part-time assistant-ships in various principal investigator labs.
Various departments at The George Washington
University Medical Center offer a wide variety of research opportunities
in various topics and concentrations.
The M.S. Degree Program in Genomics and Bioinformatics
will utilize several new pieces of equipment at the core facility.
- 2 Mass Spectrometers
- ThermoFinnigan-LCQ (ESI-ion trap)
- Kratos/Shimadzu Axima-CFR (MALDI-TOF)
- Affymetrix Microarray
- GeneChip Fluidics Station 450
- GeneChip Hybridization Oven
- GeneArray Scanner 3000
- DNA sequencer
- Peptide Sequencer
- Peptide Synthesizer
MS Student Publications from the Genomics and Bioinformatics program (2002-2007)
1. Ruaño G, Thompson PD, Windemuth A, Seip RL, Dande A, Sorokin A,
Kocherla M, Smith A, Holford TR, Wu AH. Physiogenomic association of
statin-related myalgia to serotonin receptors. Muscle Nerve. 2007
Sep;36(3):329-35.
2. Kehn K, Berro R, Alhaj A, Bottazzi ME, Yeh WI, Klase Z, Van Duyne
R, Fu S, Kashanchi F. Functional consequences of cyclin D1/BRCA1
interaction in breast cancer cells. Oncogene. 2007 Aug
2;26(35):5060-9.
3. Stepp MA, Liu Y, Pal-Ghosh S, Jurjus RA, Tadvalkar G, Sekaran A,
Losicco K, Jiang L, Larsen M, Li L, Yuspa SH. Reduced migration,
altered matrix and enhanced TGFbeta1 signaling are signatures of
mouse keratinocytes lacking Sdc1. J Cell Sci. 2007 Aug 15;120(Pt
16):2851-63. Epub 2007 Jul 31.
4. Cahan P, Rovegno F, Mooney D, Newman JC, St Laurent G 3rd,
McCaffrey TA. Meta-analysis of microarray results: challenges,
opportunities, and recommendations for standardization. Gene. 2007
Jul 3; [Epub ahead of print].
5. Klase Z, Kale P, Winograd R, Gupta MV, Heydarian M, Berro R,
McCaffrey T, Kashanchi F. HIV-1 TAR element is processed by Dicer to
yield a viral micro-RNA involved in chromatin remodeling of the
viral LTR. BMC Mol Biol. 2007 Jul 30;8(1):63 Epub ahead of print.
6. Brody F, Hill S, Celenski S, Kar R, Kluk B, Pinzone J, Fu S.
Expression of ectonucleotide pyrophosphate phosphodiesterase and
peroxisome proliferator activated receptor gamma in morbidly obese
patients. Surg Endosc. 2007 Jun;21(6):941-4.
7. Mody M, Dharker N, Bloomston M, Wang PS, Chou FS, Glickman TS,
McCaffrey T, Yang Z, Pumfery A, Lee D, Ringel MD, Pinzone JJ.
Rosiglitazone sensitizes MDA-MB-231 breast cancer cells to anti-tumour
effects of tumour necrosis factor-{alpha}, CH11 and CYC202. Endocr
Relat Cancer. 2007 Jun;14(2):305-15.
8. Xiao S, Zhan B, Xue J, Goud GN, Loukas A, Liu Y, Williamson A,
Liu S, Deumic V, Hotez P. The evaluation of recombinant hookworm
antigens as vaccines in hamsters (Mesocricetus auratus) challenged
with human hookworm, Necator americanus. Exp Parasitol. 2007 Jun 6;
[Epub ahead of print].
9. Kajdacsy-Balla A, Geynisman JM, Macias V, Setty S, Nanaji NM,
Berman JJ, Dobbin K, Melamed J, Kong X, Bosland M, Orenstein J,
Bayerl J, Becich MJ, Dhir R, Datta MW; Cooperative Prostate Cancer
Tissue Resource. Practical aspects of planning, building, and
interpreting tissue microarrays: the Cooperative Prostate Cancer
Tissue Resource experience. J Mol Histol. 2007 May;38(2):113-21.
10. Ruaño G, Goethe JW, Caley C, Woolley S, Holford TR, Kocherla M,
Windemuth A, de Leon J. Physiogenomic comparison of weight profiles
of olanzapine- and risperidone-treated patients. Mol Psychiatry.
2007 May;12(5):474-82.
11. Ruaño G, Blair CL, Bower B, Windemuth A, Kocherla M, Aleman Y,
Pearlson G, Goethe JW, Schwartz HI. Somatic complications of
psychotropic medications in a patient with multiple CYP2 drug
metabolism deficiencies. Conn Med. 2007 Apr;71(4):197-200.
12. Lechuga CG, Hernández-Nazara ZH, Hernández E, Bustamante M,
Desierto G, Cotty A, Dharker N, Choe M, Rojkind M. PI3K is involved
in PDGF-beta receptor upregulation post-PDGF-BB treatment in mouse
HSC. Am J Physiol Gastrointest Liver Physiol. 2006
Dec;291(6):G1051-61.
13. Yang AX, Mejido J, Bhattacharya B, Petersen D, Han J, Kawasaki
ES, Puri RK. Analysis of the quality of contact-pin fabricated
oligonucleotide microarrays. Mol Biotechnol. 2006 Nov;34(3):303-15.
14. de la Fuente C, Gupta MV, Klase Z, Strouss K, Cahan P, McCaffery
T, Galante A, Soteropoulos P, Pumfery A, Fujii M, Kashanchi F.
Involvement of HTLV-I Tax and CREB in aneuploidy: a bioinformatics
approach. Retrovirology. 2006 Jul 5;3:43.
15. Terwilliger DP, Buckley KM, Mehta D, Moorjani PG, Smith LC.
Unexpected diversity displayed in cDNAs expressed by the immune
cells of the purple sea urchin, Strongylocentrotus purpuratus.
Physiol Genomics. 2006 Jul 12;26(2):134-44.
16. Ruaño G, Windemuth A, Kocherla M, Holford T, Fernandez ML,
Forsythe CE, Wood RJ, Kraemer WJ, Volek JS. Physiogenomic analysis
of weight loss induced by dietary carbohydrate restriction. Nutr
Metab (Lond). 2006 May 15;3:20.
17. Fujiwara RT, Loukas A, Mendez S, Williamson AL, Bueno LL, Wang
Y, Samuel A, Zhan B, Bottazzi ME, Hotez PJ, Bethony JM. Vaccination
with irradiated Ancylostoma caninum third stage larvae induces a Th2
protective response in dogs. Vaccine. 2006 Jan 23;24(4):501-9.
18. Ruaño G, Thompson PD, Windemuth A, Smith A, Kocherla M, Holford
TR, Seip R, Wu AH. Physiogenomic analysis links serum creatine
kinase activities during statin therapy to vascular smooth muscle
homeostasis. Pharmacogenomics. 2005 Dec;6(8):865-72.
19. Curtin BF, Tetz LM, Compton JR, Doctor BP, Gordon RK, Nambiar
MP. Histone acetylase inhibitor trichostatin A induces
acetylcholinesterase expression and protects against organophosphate
exposure. J Cell Biochem. 2005 Nov 1;96(4):839-49.
20. Bhattacharya B, Cai J, Luo Y, Miura T, Mejido J, Brimble SN,
Zeng X, Schulz TC, Rao MS, Puri RK. Comparison of the gene
expression profile of undifferentiated human embryonic stem cell
lines and differentiating embryoid bodies. BMC Dev Biol. 2005 Oct
5;5:22.
21. Cahan P, Ahmad AM, Burke H, Fu S, Lai Y, Florea L, Dharker N,
Kobrinski T, Kale P, McCaffrey TA. List of lists-annotated (LOLA): a
database for annotation and comparison of published microarray gene
lists. Gene. 2005 Oct 24;360(1):78-82.
22. Zhan B, Liu S, Perally S, Xue J, Fujiwara R, Brophy P, Xiao S,
Liu Y, Feng J, Williamson A, Wang Y, Bueno LL, Mendez S, Goud G,
Bethony JM, Hawdon JM, Loukas A, Jones K, Hotez PJ. Biochemical
characterization and vaccine potential of a heme-binding glutathione
transferase from the adult hookworm Ancylostoma caninum. Infect
Immun. 2005 Oct;73 (10):6903-11.
23. Goud GN, Bottazzi ME, Zhan B, Mendez S, Deumic V, Plieskatt J,
Liu S, Wang Y, Bueno L, Fujiwara R, Samuel A, Ahn SY, Solanki M,
Asojo OA, Wang J, Bethony JM, Loukas A, Roy M, Hotez PJ. Expression
of the Necator americanus hookworm larval antigen Na-ASP-2 in Pichia
pastoris and purification of the recombinant protein for use in
human clinical trials. Vaccine. 2005 Sep 15;23(39):4754-64.
24. Yang AX, Mejido J, Luo Y, Zeng X, Schwartz C, Wu T, Thies RS,
Bhattacharya B, Han J, Freed B, Rao M, Puri RK. Development of a
focused microarray to assess human embryonic stem cell
differentiation. Stem Cells Dev. 2005 Jun;14(3):270-84.
25. Fujiwara RT, Bethony J, Bueno LL, Wang Y, Ahn SY, Samuel A,
Bottazzi ME, Hotez P, Mendez S. Immunogenicity of the hookworm
Na-ASP-2 vaccine candidate: characterization of humoral and cellular
responses after vaccination in the Sprague Dawley rat. Hum Vaccin.
2005 May-Jun;1(3):123-8. Epub 2005 May 20.
26. Mendez S, Zhan B, Goud G, Ghosh K, Dobardzic A, Wu W, Liu S,
Deumic V, Dobardzic R, Liu Y, Bethony J, Hotez PJ. Effect of
combining the larval antigens Ancylostoma secreted protein 2 (ASP-2)
and metalloprotease 1 (MTP-1) in protecting hamsters against
hookworm infection and disease caused by Ancylostoma ceylanicum.
Vaccine. 2005 May 2;23(24):3123-30.
27. Gallagher-Smith M, Kim J, Al-Bawardy R, Josko D. Francisella
tularensis: possible agent in bioterrorism. Clin Lab Sci. 2004
Winter;17(1):35-9.
28. Zhan B, Wang Y, Liu Y, Williamson A, Loukas A, Hawdon JM, Xue
HC, Xiao SH, Hotez PJ. Ac-SAA-1, an immunodominant 16 kDa
surface-associated antigen of infective larvae and adults of
Ancylostoma caninum. Int J Parasitol. 2004 Aug;34(9):1037-45.
29. Goud GN, Zhan B, Ghosh K, Loukas A, Hawdon J, Dobardzic A,
Deumic V, Liu S, Dobardzic R, Zook BC, Jin Q, Liu Y, Hoffman L,
Chung-Debose S, Patel R, Mendez S, Hotez PJ. Cloning, yeast
expression, isolation, and vaccine testing of recombinant
Ancylostoma-secreted protein (ASP)-1 and ASP-2 from Ancylostoma
ceylanicum. J Infect Dis. 2004 Mar 1;189(5):919-29.
30. Zhan B, Liu Y, Badamchian M, Williamson A, Feng J, Loukas A,
Hawdon JM, Hotez PJ. Molecular characterisation of the Ancylostoma-secreted
protein family from the adult stage of Ancylostoma caninum. Int J
Parasitol. 2003 Aug;33(9):897-907.
31. Basavaraju S, Zhan B, Kennedy MW, Liu Y, Hawdon J, Hotez PJ.
Ac-FAR-1, a 20 kDa fatty acid- and retinol-binding protein secreted
by adult Ancylostoma caninum hookworms: gene transcription pattern,
ligand binding properties and structural characterisation. Mol
Biochem Parasitol. 2003 Jan;126(1):63-71.
Manuscripts that are either in preparation or already submitted
1. Rheey J, et al. Cytokine Activity of Homeotic Transcription
Factors in Human Breast Cancer. 2007-2008.
2. Heydarian M, et al. Identification and characterization of novel
splice variants of sFlt in preeclampsia. 2007-2008.
3. Kanakabandi K, et al. Stress, Stress Induced Premature Senescence
and Role of micro RNAs in Hydrogen Peroxide induced Stress.
2007-2008.
4. Ribble R, et al. Compositional and Structural Analysis of
Distinct IKK Complexes in HTLV-1 Infected Cells. 2007-2008.
5. Kim K, et al. Identification of Potential Biomarkers of Autism
Spectrum Disorders (ASD) In Lymphoblastoid Cell Lines of Affected
Individuals. 2007-2008.
6. Van Duyne R, et al. The Dissociation of the HIV-1 cdk2/Cyclin E
Complex with Tat Derived Small Peptide Inhibitors. 2007-2008.
7. Mussar K, et al. Utilization of Pattern Based Discovery: A
Bioinformatic Algorithmic Approach to Discovering novel miRNAs and
their Target Sites. 2007-2008.
8. Gindin Y, et al. Genetic Analysis of the IL-12 Receptor in the
C57BL/6 and the BALB/c Inbred Mouse Strains. 2007-2008.
9. Lee H, et al. TLX1 Protein-Protein Interaction with CBP, TLE1 and
PP1 in T Cell Acute Lymphoblastic Leukemia. 2007-2008.
10. Ratnayake S, et al. Haemophilus influenzae Comparative Genomics.
2007-2008.
11. Faghfoory A, et al. Expression of the Glutamic Acid
Decarboxylase (GAD) Genes in Arabidopsis thaliana in Response to
Mechanical Damage, Cold, Heat. 2007-2008.
12. Seelamgari A, et al. Role of Viral Regulatory and Accessory
Proteins in HIV-1 Replication. 2007-2008.
13. Akpan U, et al. The Role of Cyclin D2 and Acting in Human T-Cell
Leukemia Virus Type-1 Infected Cells. 2007-2008.
14. An E, et al. Proteomics of Beta B1-Crystallin. 2007-2008.
15. Miller N, et al. Development of a High-throughput Pipeline for
the Decoding of Influenzavirus Genomic RNA. 2007-2008.
16. Cahan P, et al. Adapting and Expanding Open Source Microarray
Data Analysis Software for the Affymetrix Platform. 2007-2008.
17. Mussar K, et al. Novel miRNA detetion and miRNA target
prediction through pattern based recognition. 2007-2008
18. Krishna Banaudha, et al. Role of cellular microRNA (miR-141 and
miR-200a) in HCV viral replication in long term human hepatocyte
culture. 2007-2008
19. B.Lin, et al. Analysis of chemotherapy response program using
MPSS and SBS technology. 2007-2008
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